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Clinical Biochemistry

Responsible teacher: assoc. prof. RNDr. Eva Uhlíková, PhD.

Contact person: eva.uhlikova@fmed.uniba.sk

Examination of the subject Clinical Biochemistry in the school year 2021/2022

If the situation does not change, we write the test in person (not online).

Students are waiting for the exam at STU, Sasinkova 2, on the 2nd floor in front of the lecture hall of the Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry. You need to bring an ID card or ISIC card for identification.

Since there is also a calculation task in the test, you need to have a calculator. Mobile phones must not be used.

Test structure for Clinical Biochemistry exam

The whole test has 40 questions.

Each of the first 32 questions has four possible answers, and it is necessary to evaluate each possible answer whether it is correct or incorrect. Each answer is evaluated and the student receives one point for the correct answer. When the whole question is solved correctly, it is possible to get 4 points. As a result, a maximum of 128 points (32 x 4 = 128) can be obtained for this part of the test.

In the next eight questions of the test, the correct answer must be written.

  • Two questions are the cases of a patient with an disorder of acid-base balance. pH, pCO2 and HCO3 data are entered, or some other laboratory data, and your task is to write down what type of disorder the patient has.
  • The other two questions are for calculation. LDL-cholesterol concentration and creatinine clearance should be calculated. You need to bring a calculator, as mobile phones must not be used.
  • One question is to indicate a suitable oncomarker for a particular type of tumor.
  • One question is to diagnose the type of endocrine disorder or the type of proteinuria based on the given laboratory parameters.
  • In two questions it is necessary to write reference values for a certain laboratory parameter.

4 points can be obtained for the correct solution of each of these eight questions. It is therefore possible to obtain a maximum of 32 points for this part of the test.

A maximum of 160 points can be obtained for the correct solution of the whole test. A minimum of 129 points is required to pass the exam. The time to write the test is 50 minutes

Program of lectures

Programme of the subject: CLINICAL BIOCHEMISTRY

Study branch: GENERAL MEDICINE – 4th year Medical faculty UK

L E C T U R E S

Winter semester – academic year 2020/2022

 

 

Date

Topic of the lecture

Lecturer

1.

General clinical biochemistry – instruction, laboratory examination and its phases, pre-analytic, analytic and post-analytic phase. Diagnostic criteria of laboratory test. Reference values and evaluation of laboratory tests results.

doc. Uhlíková

2.

Plasma proteins in diagnostics. Hyper- and hypoproteinaemia. Monoclonal gammopathies and their diagnosis.

doc. Uhlíková

3.

Fundamentals of clinical enzymology. Examination of enzyme activities in diagnostics.

doc. Uhlíková

4.

Lipid parameters in diagnostics. Hyperlipoproteinemia’s and their classification. Diagnosis of lipid metabolism disorders.

doc. Uhlíková

5.

Laboratory tests in diabetology. Diagnosis and monitoring of a patient with diabetes mellitus. Diabetic complications.

doc. Uhlíková

6.

Laboratory tests in gastroenterology. Gastric examination, examination of pancreatic function and small intestine resorption disorders.

doc. Uhlíková

7.

Laboratory tests in hepatology. Examination of liver disorders and hepatic function. Cholestasis and its diagnosis.

doc. Uhlíková

8.

Laboratory tests in nephrology. Urine examination – physical, chemical and microscopic. Examination of glomerular filtration and tubular function.

doc. Uhlíková

9.

Laboratory tests in cardiology. Myocardial ischemia and diagnostics of acute myocardial infarction. Risk factors of atherosclerosis.

doc. Uhlíková

10.

Laboratory tests in cancer patients. Tumour markers and their use in the diagnosis and monitoring of patients with oncological diseases.

doc. Uhlíková

11.

Laboratory tests for some endocrinological diseases. Examination of thyroid function and function of adrenal cortex.

doc. Uhlíková

12.

Acid-base balance disorders and their diagnostics. Acidosis, alkalosis and combined acid-base balance disorders.

doc. Uhlíková

13.

Investigation of less common biological fluids – cerebrospinal fluid, synovial fluid and amniotic fluid.

doc. Uhlíková

Reference values required for the test

parameter

values

parameter

values

Hemoglobin

135-175 g/L    M

115-160 g/L    F

Albumin

35-53 g/L

Hematocrit

39-51%   M

33-42%   F

Urea in serum

2.5-8.3 mmol/L

Erythrocytes

4.2-5.7x1012/L   M

3.5-5.1x1012/L   F

Uric acid

140-420 µmol/L

Leukocytes

4.1-10.2x109/L

Creatinine

40-110 µmol/L

Thrombocytes

150-400x109/L

Total bilirubin

to 20 µmol/L

Total proteins

60-80 g/L

Conjugated bilirubin

to 5 µmol/L

Sodium

132-145 mmol/L

Triglycerides

0.7-1.9 mmol/L

Potassium

3.8-5.2 mmol/L

Total cholesterol

3.8-5.2 mmol/L

Chlorides

97-108 mmol/L

HDL-cholesterol

F    ˃  1.6 mmol/L

M   ˃ 1.4 mmol/L

Calcium

2.25-2.75 mmol/L

LDL-cholesterol

<  3.4 mmol/L

Glycemia

3.3-5.6 mmol/L

Glycated hemoglobin

< 4 mmol/L

Iron

14.3-26.0 µmol/L   M

10.7-21.5 µmol/L   F

Serum phosphates

0.7-1.6 mmol/L